Metrolab Blog

Reverse phase analysis of free complex glycans

In the last few years, biopharmaceutical analysis of complex glycans released from native proteins or protein/ antibody based drugs (removal by PNGase F) has become a standard step in full protein characterization. This step involves the separation of the glycans and analysis by high resolution accurate mass analysis or matching retention times to known standards by UV/CAD/Fluorescence.
Thermo Fisher Scientific has three powerful columns for glycan analysis: Thermo Scientific™ GlycanPac™ AXH-1 and Thermo Scientific™ Accucore™ Amide-HILIC for performing hydrophillic interaction chromatography and the Thermo Scientific™ GlycanPac™ AXR-1 a reverse phase column.
In this application brief you can find useful information about the analysis of free complex glycans using GlycanPac™ AXR-1

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