Whilst historically it was the earliest form of HPLC, normal phase separations have recently received less attention due to the belief that it is complicated and unpredictable. However, normal phase chromatography is a powerful tool for the separation of positional isomers that are difficult to separate in reversed phase mode. Due to a rigid surface, compared to the more flexible carbon chains of reversed phases, the analytes are influenced by well-defined steric interaction with polar groups.
YMC offers columns packed with non-bonded silica or with silica gel modified with polar groups.
Available Phases are:
YMC-Pack SIL, YMC-Pack PVA-Sil, YMC-Pack CN, YMC-Pack Diol-NP, YMC-Pack Polyamine II, YMC-Pack NH2, YMC-Pack TMS
For more information click here.
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