Despite advances in analytical detection technology, achieving required limits of sensitivity can still be an issue for many bioanalytical laboratories. In order to improve limits of detection analysts are looking to sample preparation in order to pre-concentrate their sample prior to analysis.
Traditional scale SPE helps to clean up the sample to minimize matrix effects, however in order to pre concentrate the sample a lengthy dry down and reconstitution step needs to be employed. This process is not only time consuming but can have a detrimental effect on the recovery of the analyte due to volatility or non specific binding.
Thermo Scientific SOLAμ products allow users to preconcentrate the sample up to 20 times prior to injection, allowing greater limits of sensitivity to be achieved whilst maintaining a high level of analyte recovery, accuracy and precision.
This application note demonstrates the use of Thermo Scientific™ SOLAμ™ Solid Phase Extraction (SPE) product to enhance sample pre-concentration prior to analysis.
Additional benefits include reduced workflow and stability for analytes susceptible to loss or degradation during evaporation and reconstitution. The use of a Thermo Scientific™ Accucore™ HPLC column provided fast and efficient separation without the need for an ultra high pressure system. MS/MS detection was performed on a Thermo Scientific™ TSQ Vantage™ mass spectrometer.
Download the application note here.