Polycyclic aromatic hydrocarbons (PAHs) are a group of chemicals formed from the incomplete combustion of organic matter. Due to their potential carcinogenic and mutagenic properties, most countries have regulations limiting the concentrations of a variety of PAHs in drinking water, food additives, cosmetics, workplaces, and factory emissions.
Thermo Fisher Scientific scientists present an Application Note which provides on-line SPE HPLC methods to quantify low concentrations of PAHs in oil. The costs for the SPE cartridge, labor, time, and reagents are significantly reduced using these methods, and the results are more consistent. This is because on-line SPE eliminates manual processes, such as rotary evaporation and nitrogen-assisted evaporation in the routine liquid-liquid extraction and offline SPE steps described in EPA Methods 550, 550.1, and 610. However, because the run time of the reported on-line SPE HPLC method exceeded 60 min in this study, the analytical column used in the AN was replaced to shorten analysis time.
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