Metrolab Blog

NDF Determination in Feed (Van Soest method)

ISO 16472:2006 Animal feeding stuffs — Determination of amylase-treated Neutral Detergent Fibre content (aNDF)
AOAC 2002.04 Amylase-treated Neutral Detergent Fibre in Feeds

Forage quality is a direct reflection of its essential nutrient content and availability to the consuming animal.
The concept behind the detergent fiber analysis (NDF) is that plant cell substances can be divided into less digestible cell walls (made of hemicellulose, cellulose and lignin) and the highly digestible cell contents (containing starch and sugars).
Hemicellulose, cellulose and lignin are indigestible in non-ruminants, while hemicellulose and cellulose are partially digestible in ruminants. NDF is a good indicator of the “bulk” fiber and has been used to predict feed intake, in other words, how much an animal will eat before its stomach is full and it stops eating.
The amylase-treated NDF method, therefore, was developed as an accurate and precise method of measuring total insoluble fiber in feeds.
The performance of VELP FIWE Advance Fiber AutoExtractor was evaluated by participating in the proficiency testing program organized by BIPEA. Samples were analyzed using FIWE Advance Fiber analyzer and the obtained results were compared with the BIPEA tolerance range.

Download the Application Note of FIWE Advance showing the results of NDF in feed.