NanoLC-MS has long been considered the mainstream methodology in proteomics-based research due to its high sensitivity. While increased ease-of-use and robustness are essential to its widespread adoption, the transition from academic research to industrial or clinical research applications is equally dependent upon achieving the highest levels of both intra and inter-system reproducibility.
This study demonstrates how the combined power of the Vanquish Neo UHPLC system, accompanying PepMap Neo columns and the latest HRAM Orbitrap mass spectrometers deliver the level of system-to-system reproducibility required to meet the demands of large cohort and multi center studies. In particular, the capacity of the Vanquish Neo UHPLC system to deliver standardized, rugged, and reproducible analytics will help foster the adoption of nanoLC-MS for large sample cohorts analysis.
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