In this case study two different major method development parameters for peptides in RP-HPLC are introduced:
1. Temperature
2. Mobile phase
The following example shows three defensin peptides, which are very similar as they differ only in one amino acid on the N-terminal:
α-Defensin-1: MW 3,442
α-Defensin-2: MW 3,371
α-Defensin-3: MW 3,486
You will find out here, how to separate these three forms from each other with an easy UHPLC-C18 method.
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