Metrolab Blog

Tips for Separating Peptides

In this case study two different major method development parameters for peptides in RP-HPLC are introduced:

1. Temperature 
2. Mobile phase
The following example shows three defensin peptides, which are very similar as they differ only in one amino acid on the N-terminal:
α-Defensin-1: MW 3,442
α-Defensin-2: MW 3,371
α-Defensin-3: MW 3,486
You will find out here, how to separate these three forms from each other with an easy UHPLC-C18 method.
In order to learn more about peptide separations, please download your personal copy of the document.