This application note demonstrated that the AOAC 2018.16 First Action Method for sugar determination in food samples could be successfully modified for use with a Dionex CarboPac PA20-1 mm column and HPAE-PAD in Dual Eluent Generation Cartridge Mode. The separation, linearity, sensitivity, accuracy, and reproducibility were excellent.
Compared with a traditional HPAE-PAD separation of sugars using manually prepared sodium hydroxide eluents, the Dual EGC method delivered a similar resolution of sugars, simplified operation (no eluent preparation), and improved retention time precision.