Choosing the correct pipetting technique helps securing the accuracy and precision necessary for reproducible, reliable results. Especially when pipetting small volumes the influence of the pipetting technique can have tremendous effects on the experimental result. Two main techniques exist and each should be applied depending on the sample liquid.
Aqueous liquids can be dispensed using the well-known forward pipetting technique, but dispensing challenging liquids like glycerol, ethanol or BSA solution need a different approach called reverse pipetting. Additionally general considerations like a low immersion depth and a vertical pipetting angle must be taken into account. Learn about proper pipetting techniques and their individual advantages in our Userguide. Forward pipetting is the standard technique for most aqueous solutions.
Reverse pipetting is recommended for viscous or foaming liquids as well as very small volumes. The blow-out volume is additionally aspirated in the first step and stays in the pipette tip to be discarded.
The impact of immersion depth and angle on precision and accuracy is huge. A vertical angle and low immersion depth guarantee the most accurate pipetting result.
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