Thermo Scientific™ Q Exactive™ hybrid quadrupole-Orbitrap™ series mass spectrometers offer analysts a range of scan modes that provide functionality comparable to the modes provided by conventional tandem quadrupole mass spectrometers: the product ion, fragment ion, and data-dependent neutral loss trigger scan modes.
Although the Q Exactive series mass spectrometers provide the analyst with a toolbox similar to that of triple quadrupole (QQQ) instruments, key differences exist between the two technologies.
Most importantly, the Orbitrap analyzer of the Q Exactive series mass spectrometer is a trapping, not a scanning, device. Certain Q Exactive series mass spectrometer data acquisition modes may resemble conventional QQQ scan modes, but there are differences in their selectivity and sensitivity characteristics, as well as the number of data points acquired per unit time.
This white paper describes each of the various scan modes, and the key
differences between trapping and scanning devices. Method development
guidance, including consideration of intrinsic dependencies such as
resolution and scan speed, sensitivity, and selectivity, is also provided .
Find the application note here
Authors :
Anton Kaufmann,
KLZH, Zürich, Switzerland
Maciej Bromirski,
Thermo Fisher Scientific,
San Jose, CA, USA
