Vitamin K1 (phylloquinone) and vitamin K2 (group of compounds; menaquinones) are crucial for their roles in controlling blood clotting and regulation of bone metabolism. These fat-soluble vitamins are problematic for typical reversed-phase liquid chromatography as they are very lipophilic and often suffer from matrix interferences from co-eluting phospholipids. Typically, complex, multistep sample preparation is employed meaning a lengthy analysis time. Here, a simplified sample preparation approach was explored and shown to be suitable for clinical research when coupled with a Thermo Scientific™ Accucore™ Biphenyl analytical column providing excellent separation from endogenous matrix components. High performance liquid chromatography was achieved on the Thermo Scientific™ Vanquish™ Horizon UHPLC system with excellent retention time stability. Detection was provided by the Thermo Scientific™ TSQ Endura™ triple-stage quadrupole mass spectrometer.
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