Hypersil GOLD Peptide column for reliable monitoring of glycopeptide variants in monoclonal antibodies by LC-UV-MS analysis

Authors

Sylvia Grosse, Michaela S. Scherz , Serdar Bilgesoy

Application benefits

• Thermo Scientific™ Hypersil GOLD™ Peptide columns help achieve accurate and reproducible results when monitoring glycopeptide variants in mAb digest samples.
• Hypersil GOLD Peptide columns exhibit low column-to-column and lot-to-lot variability, resulting in consistent performance when analyzing glypeptides in mAb samples.

Goal

Demonstrating the benefits of the Hypersil GOLD Peptide C18 column in LC-UV-SQMS
analysis of tryptic mAb digest samples.

Introduction

Therapeutic monoclonal antibodies (mAbs) are recombinant proteins produced by
highly complex ectopic expression in cell culture and used in biotherapeutics. They
are susceptible to various chemical and enzymatic modifications during expression,
purification, and long-term storage.

Read all the application note here.

See also,

Application Note: A comparison of reversed-phase C18 columns for peptide mapping of monoclonal antibodies

Flyer: Resolve all your peptide Challenges

Manual: man-014-ccs-lc-Hypersil-GOLD-Peptide-man014-en