- The chemical reaction for the determination of the substance must be known.
- The optimal wavelength for the measurement must be known, otherwise a spectrum must be recorded to indicate maximum peak or suitable λ.
- Setting up a dilution series for e.g. a 10-point calibration: A dilution series in duplicate achieves a higher accuracy.
- After reaction and reading, the absorbance values are plotted against the concentration values.
Today, photometers offer user-guided functions to set-up user-defined or pre-programmed calibration
- General settings for cuvette size, measurement range, wavelengths etc.
- Reading of dilution solutions including calculation of the mean values of duplicates.
- Alternatively, entering simply the slope factor.
- Blank values (E0) can be stored.
- Calibration characteristics are stored with a method (program) identification (number or name)
- Automatic setting of wavelength and measuring range during method selection via list or Barcode.
- Switching citations and units
- Setting for AQA default values for standard solutions and checking intervals
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