Metrolab Blog

Column Selection for Reversed-Phase HPLC

There are many factors that influence the performance of a high performance liquid chromatography (HPLC) stationary phase, of which the chemical nature of the bonded phase ligand is important, but by no means all encompassing. Minor manufacturing parameters such as the method of electropolishing the internal surface of the column can also have an effect on the selectivity and efficiency produced by a particular column.
Several attempts have been made to produce a “definitive” set of chemical probes to best characterize the huge number of stationary phases available (well over 1000 different types are currently available). As yet a harmonized set of test probes and methodologies has not been identified, however three, independent, publicly available databases of HPLC columns exist today:

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