Metrolab Blog

ELISA (enzyme-linked immunosorbent assay)

The enzyme-linked immunosorbent assay (ELISA) procedure is ubiquitous in biomedical research and clinical testing. ELISA, first described by Engvall and Perlmann in 1971, is an antibody-based reaction that...

Particle Counters Knowledge Hub 13

𝗔𝗶𝗿 𝗙𝗹𝗼𝘄 𝗩𝗲𝗹𝗼𝗰𝗶𝘁𝘆 𝗖𝗮𝘀𝗲 : 0.45𝘮/𝘴, 𝘣𝘶𝘵 𝘧𝘳𝘰𝘮 𝘸𝘩𝘦𝘳𝘦? 🔠 Even all regulations/standards (ISO14644-3, GMP Annex1, US FDA, GMP, etc.) agree on 0.45m/s ±20% air velocity, they are giving a different perspective on...

Particle Counters keep in mind 11

𝗖𝗹𝗲𝗮𝗻𝗿𝗼𝗼𝗺 𝗖𝗹𝗮𝘀𝘀𝗶𝗳𝗶𝗰𝗮𝘁𝗶𝗼𝗻: 𝗩𝘀 «𝗠𝗶𝗻𝗶𝗺𝘂𝗺 𝗦𝗮𝗺𝗽𝗹𝗲 𝗗𝘂𝗿𝗮𝘁𝗶𝗼𝗻 »Table showing minimum duration (minutes) if you are using 1 CFM (28.3LPM) Particle Counters. 🔠According to ISO 14644-1:2015; ☑ If concentrations are...

Particle Counters keep in mind 10

𝗜𝗦𝗢 𝟭𝟰𝟲𝟰𝟰-𝟯:𝟮𝟬𝟭𝟵 𝗖𝗹𝗲𝗮𝗻𝗿𝗼𝗼𝗺 𝗧𝗲𝘀𝘁 𝗠𝗲𝘁𝗵𝗼𝗱𝘀; 𝗦𝘂𝗽𝗽𝗼𝗿𝘁𝗶𝗻𝗴 𝗧𝗲𝘀𝘁𝘀 ISO 14644-1 shall be carried out in order to classify a cleanroom by airborne particle concentration. ISO 14644-3 Table 2 lists other appropriate tests...

Particle Counters Knowledge Hub 12

𝙃𝙤𝙬 𝙩𝙤 𝙏𝙚𝙨𝙩 𝙄𝙣𝙨𝙩𝙖𝙡𝙡𝙚𝙙 𝙃𝙀𝙋𝘼 𝙁𝙞𝙡𝙩𝙚𝙧 𝙄𝙣𝙩𝙚𝙜𝙧𝙞𝙩𝙮❓ ☑ Measure the aerosol concentration upstream of the filters ☑This aerosol concentration should be used as the upstream 100 % reference for the photometer...

Particle Counters Knowledge Hub 11

Claim: HEPA filters cannot capture viruses like coronavirus because efficiency is based on 0.3micron and coronavirus is about 0.01micron Reality; This is not a true statement. Filters are a combination of...

Particle Counters keep in mind 9

𝗦𝗲𝗹𝗲𝗰𝘁𝗶𝗻𝗴 𝗮 𝗣𝗮𝗿𝘁𝗶𝗰𝗹𝗲 𝗖𝗹𝗮𝘀𝘀𝗶𝗳𝗶𝗰𝗮𝘁𝗶𝗼𝗻 𝗟𝗼𝗰𝗮𝘁𝗶𝗼𝗻𝘀 𝗶𝗻 𝗖𝗿𝗶𝘁𝗶𝗰𝗮𝗹 𝗭𝗼𝗻𝗲𝘀 According to EU GMP Annex1 and ISO 14644-1, selection of sampling locations inside critical zones are defined as; 𝗘𝗨 𝗚𝗠𝗣 𝗔𝗡𝗡𝗘𝗫𝟭 𝟮𝟬𝟮𝟬 (𝗗𝗿𝗮𝗳𝘁)«4.30...

Particle Counters keep in mind 8

𝗖𝗹𝗲𝗮𝗻𝗿𝗼𝗼𝗺 𝗖𝗹𝗮𝘀𝘀𝗶𝗳𝗶𝗰𝗮𝘁𝗶𝗼𝗻 𝗩𝘀. 𝗖𝗹𝗲𝗮𝗻𝗿𝗼𝗼𝗺 𝗠𝗼𝗻𝗶𝘁𝗼𝗿𝗶𝗻𝗴 The most confusing terms in cleanrooms; classification requirements and online monitoring. The below table shows the differences in terms of regulations...

Particle Counters Knowledge Hub 10

𝗪𝗵𝗮𝘁 𝘀𝗵𝗼𝘂𝗹𝗱 𝗯𝗲 𝗶𝗻𝗰𝗹𝘂𝗱𝗲𝗱 𝗶𝗻 𝘁𝗵𝗲 𝗰𝗹𝗲𝗮𝗻𝗿𝗼𝗼𝗺 𝗰𝗹𝗮𝘀𝘀𝗶𝗳𝗶𝗰𝗮𝘁𝗶𝗼𝗻 𝘁𝗲𝘀𝘁 𝗿𝗲𝗽𝗼𝗿𝘁❓ #Cleanroom particle #classification test report shall include; ✔𝘁𝗵𝗲 𝗻𝗮𝗺𝗲 𝗮𝗻𝗱 𝗮𝗱𝗱𝗿𝗲𝘀𝘀 𝗼𝗳 𝘁𝗵𝗲 𝘁𝗲𝘀𝘁𝗶𝗻𝗴 𝗼𝗿𝗴𝗮𝗻𝗶𝘇𝗮𝘁𝗶𝗼𝗻, and the...

Particle Counters keep in mind 7

𝗜𝗦𝗢 𝟭𝟰𝟲𝟰𝟰-𝟯:𝟮𝟬𝟭𝟵 𝗖𝗹𝗲𝗮𝗻𝗿𝗼𝗼𝗺 𝗧𝗲𝘀𝘁 𝗠𝗲𝘁𝗵𝗼𝗱𝘀; 𝗦𝘂𝗽𝗽𝗼𝗿𝘁𝗶𝗻𝗴 𝗧𝗲𝘀𝘁𝘀 ISO 14644-1 shall be carried out in order to classify a cleanroom by airborne particle concentration. ISO 14644-3 Table 2 lists other appropriate tests...

Category - Pharma