The primary goal of a preparative liquid chromatography is the purification of target compounds through fractionation. A major secondary goal for laboratories is the method economy of this analysis. To save money, chromatographers often begin their method screening at the analytical scale, to determine the optimum separation gradient and column chemistry that delivers the resolution of their target components. Through this method design, conscious decisions are made, such as selecting methanol over acetonitrile, minimizing the use of expensive buffers, etc., to design a method that saves material cost for the laboratory.
In this application note, a compound mixture of voriconazole and several known impurities was used as a model to replicate raw pharmaceutical synthesis. Method design and column screening were performed to demonstrate the considerations a chromatographer may take as they develop a method for preparative scale chromatography.
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