Combining the Thermo Scientific™ SMART Digest™ magnetic kits with the Thermo Scientific™ KingFisher™ Duo Prime purification system provides an automated approach to protein digest method optimization.
• High quality, reproducible peptide mapping data eases the burden of method development, optimization, and validation.
• No denaturing reagents required – heat stable enzymes enable thermal denaturation.
• Immobilized trypsin in excess to sample – removes requirement to determine optimum enzyme to protease ratio.
• Precise termination of digestion – magnetic beads enable automated removal of immobilized trypsin by the KingFisher Duo Prime system.
• Downloadable Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) software eWorkflow and Thermo Scientific™ BindIt™ software time-course protocol to determine minimum optimal digest time.
Download the application note here.