In liquid chromatography, good resolution of peaks is the goal in order to gain meaningful information. Mixed-Mode chromatography provides better control of resolution, as more parameters can be adjusted in order to optimize resolution. Thermo Scientific Acclaim Mixed-Mode columns provide unique surface chemistries which allows the chromatographer to adjust the elution of analytes easily and achieve maximum resolution, often providing resolution of compounds not possible by other conventional high performance liquid chromatography (HPLC) columns. The combination of ion exchange with reversed-phase, or HILIC chemistries allows the chromatographer to exploit chemical properties of the analyte mixture to fully resolve the components of a complex sample or for sensitive detection of trace amounts of analytes. Reversed-phase chromatography alone could not accomplish this because it would not retain the very polar analytes or charged analytes. Ion-exchange (IEX) chromatography alone would be insufficient for hydrophobic compounds. Having a column chemistry with a combination of functionalities gives you a whole new tool set to resolve and evaluate your samples.
Acclaim Mixed-Mode columns are available in two functionalities (reversed-phase + ion-exchange) and three functionalities (reversed-phase or HILIC + ion-exchange). The greatest benefit of using Mixed-Mode columns is selectivity optimization, simply by adjusting mobile phase ionic strength, pH and/or organic solvent concentration. With adjustable selectivity, it is also possible to separate analytes with dramatically different hydrophobicity and charge state, such as simultaneous separation of active pharmaceutical ingredients (API) and counterion, in a single analysis.
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