In this educational back-to-school webinar, James Grinias (University of Rowan) and Frank Steiner (Thermo Scientific) discuss strategic approaches for development, optimization and transfer of legacy and modern HPLC methods, utilizing gradient elution.
Discussion topics and data on how to more easily move to core-shell column technology, how to modify gradient methods effectively, and how to work within the framework of evolving rules for method transfer such as USP 621. We also discuss using the right tool to reduce the impact of method transfer across HPLC platforms.
Key learning objectives:
Tricks and tips on method development and transfer
Understanding gradient-based LC separations
Scalable parameters in pharmacopeia method modernization