This application note describes the applicability of middle-up techniques for biotherapeutics characterization. Shows fast, efficient separation using reversed-phase chromatography with Thermo Scientific™ MAbPac™ RP columns for the characterization of mAb variants. Demonstrates the effect of column i.d. on the resolution, loading capacity, mobile phase composition, and ease of use.
Performance evaluation of MAbPac RP columns for monoclonal antibody IdeS subunit analysis
