Metrolab Blog

Track and measure the dynamic movements of your cells with the new Gen5 software module from Agilent BioTek

The concerted movement of cells within their environment plays a fundamental role in nearly all eukaryotic organisms. Higher eukaryotes rely on regulated cell migration throughout development and for immunologic processes, while aberrant cell migration is a defining feature of metastatic cancers. The process of cellular migration towards external stimuli (such as chemotaxis) is called directional cell migration. Equally as informative is random cell migration, in which cell trajectories are measured when a chemoattractant is diffuse instead of emanating from a source direction. Whether studying directional or random migration, it is important to measure the motile capacity of cells accurately and reliably. 

The Agilent BioTek Gen5 Object Tracking Module provides an automated method for characterizing 2D cell migration in high-content formats, including 96-well microplates and chemotaxis slides. Combined with Agilent BioTek automated imaging systems, this capability delivers an integrated solution for automating cell migration studies, increasing assay robustness and throughput. Below we discuss a few aspects to consider when setting up both random and direct cell migration assays.

For more information about imaging you can visit the Agilent website