Multi-Mode Metabolic Profiling of Stationary Phase Saccharomyces Cerevisiae Exposed to Short-Term Oxidative or Nutrient Stress

Due to the prevalent use of yeast as a model system, the choice of genomic, biochemical, and cell biology tools compatible with these cells is well-developed and wide-ranging. The ability to choose from a variety of assays that can combine optimal workflow productivity with relevant data outcomes can be limited by the need for multiple devices to perform different task and detection methods. Here we demonstrate a flexible and versatile instrument that supports a broad range of yeast studies, combining multiple imaging and detection modes with on-board environmental control and shaking. In this study, multiple assay formats were used to conduct a detailed characterization of oxidative and nutrient stress response in Saccharomyces cerevisiae.

(A.) BioTek’s Cytation™ 5 Cell Imaging Multi-Mode Reader has functionality that can enable microbiology workflow tasks typically requiring multiple pieces of equipment to be accomplished using only one instrument. (B.) Protocols used in this application integrate Cytation 5 features highlighted with a purple check mark in (A.). Options amenable to the procedure are shown with a (+/-) symbol.

Author: Wendy Goodrich, Applications Scientist, BioTek Instruments, Inc., Winooski, VT