Monitoring pH in Long-term Proliferation Assays

Using the Cytation 5 Cell Imaging Multi-Mode Reader to monitor Cell Culture Status Concurrently with Imaging

Abstract:Media formulations for the propagation of in vitro tissue culture often contain the pH Indicator dye phenol red. We have previously shown that phenol red absorbance can be used to determine pH in cell cultures.

In this application note we describe the use of the Cytation™ 5 Cell Imaging Multi-Mode Reader to monitor culture media pH via absorbance measurements, during multi-day imaged-based cell proliferation assays.

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