Ca2+ acts as an important second messenger in diverse signaling pathways, including G protein-coupled receptors. Characterizing these pathways requires the ability to detect rapid changes in intracellular Ca2+ levels with high temporal resolution. Here we describe a live cell imaging based approach to quantify Ca2+ flux kinetics using the Lionheart™ FX and Fluo-4 Ca2+ indicator dye that delivers sub-second resolution and a large assay window.
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