Many multi-mode microplate readers use monochromator-based optics for fluorescence intensity measurements, allowing great flexibility to use multiple fluorescent dyes and stains. Most of these systems offer a fixed bandwidth, which works well for standard fluorophores but aren’t always optimal for some others, including those with a narrow Stokes’ Shift.
Synergy™ Neo2 Multi-Mode Reader and Cytation™ 5 Cell Imaging Multi-Mode Reader have monochromator optics that incorporate user-selectable variable bandwidths for the best assay specificity and assay sensitivity. Synergy Neo2’s monochromators have a range from 3 nm to 50 nm, while Cytation 5 has a range of 9 nm to 50 nm; both are selectable in 1 nm increments.
Learn more about the benefits of variable bandwidth monochromators for fluorescence detection:
Learn more about optimizing applications using variable bandwidth in BioTek’s multi-mode microplate readers here.
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