AlphaScreen® is a bead-based assay technology used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for amplified luminescent proximity homogeneous assay. This platform provides a non-radioactive, homogeneous assay which has low background and high signal:background ratios. The assay can be easily automated and miniaturized to a variety of well formats. The assay incorporates two sets of beads, donor and acceptor beads, to generate a signal. The donor bead is illuminated at 680 nm. If the acceptor bead is in close proximity to the donor bead, energy is transferred to the acceptor bead, and light is produced in the 520-620 nm range. If an acceptor bead is not in close proximity to the donor bead, no energy is transferred, and the donor bead falls back to its ground state
Synergy™ Neo2 and Cytation™ 5 have an 100 mW laser excitation source, ideal for providing the excitation energy needed for fast and sensitive Alpha assays. Synergy HTX offers a lower cost option for Alpha assays using a Tungsten Halogen light source.
Assay principal for AlphaScreen® SureFire® STAT3 assay
See an example here.
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